Recombinant Human B7-H6 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033283-10, PKSH033283-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: B7-H6
Target Symptom: Natural cytotoxicity triggering receptor 3 ligand 1; B7 homolog 6; B7-H6; NCR3LG1; B7H6
Research Area: immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
Accession: NP_001189368.1
Background: Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.
Activity: Immobilized Human B7-H6-His at 2μg/ml(100 μl/well) can bind NCR3-Fc. The ED50 of Human B7-H6-His is 6. 40 ug/ml.
Sequence: Asp25-Ser262
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 27.5 kDa
Observed MW: 27.5 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only