Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030396-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: c-MET; HGFR
Target Symptom: AUTS9; c-Met; DFNB97; HGFR; RCCP2
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: ELISA
Fusion Tag: N-His & GST
UNIProt ID: P08581
Accession: P08581-1
Background: Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
Activity: 1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.
2. Immobilized human HGFR (aa 956-1390) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml.
Sequence: Lys956-Ser1390
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 76.8 kDa
Observed MW: 68 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only