Recombinant Human Carbonic anhydrase 12 Protein
Sizes: 20ug, 50ug, 100ug
Catalogue Numbers: RP00157-20, RP00157-50, RP00157-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Carbonic anhydrase 12 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Gln291) of human Carbonic Anhydrase XII/CA12 (Accession #NP_001209.1) fused with a 6×His tag at the C-terminus.
Alternate Names: CA12, CA-XII, CAXII, HsT18816, T18816
SWISS: O43570
GeneID: 771
Species: Human
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme that is highly expressed in normal tissues, such as colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. It has been found to be overexpressed in 10% of clear cell renal carcinomas.
Bio-Activity: Measured by its esterase activity. The specific activity is >48 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQ
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only