Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032163-10, PKSH032163-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carbonic Anhydrase 5B; CA5B
Target Synonym: Carbonic Anhydrase 5B Mitochondrial; Carbonate Dehydratase VB; Carbonic Anhydrase VB; CA-VB; CA5B
UNIProt ID: Q9Y2D0
Research Areas: Signal Transduction; Tags & Cell Markers; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: Q9Y2D0
Background: Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes; including calcification; respiration; bone resorption; acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart; pancreas; kidney; placenta; lung; and skeletal muscle; but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA; CA-VA. CA5B is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin; and Foscarnet.
Sequence: Cys34-Pro317
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33.8 kDa
Observed MW: 33 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only