Recombinant Human Carboxylesterase 1/CES1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032167-10, PKSH032167-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carboxylesterase 1; CES1
Target Synonym: Liver Carboxylesterase 1; Acyl-Coenzyme A:Cholesterol Acyltransferase; ACAT; Brain Carboxylesterase hBr1; Carboxylesterase 1; CE-1; hCE-1; Cocaine Carboxylesterase; Egasyn; HMSE; Methylumbelliferyl-Acetate Deacetylase 1; Monocyte/Macrophage Serine Esterase; Retinyl Ester Hydrolase; REH; Serine Esterase 1; Triacylglycerol Hydrolase; TGH; CES1; CES2; SES1
UNIProt ID: P23141
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P23141-3
Background: Carboxylesterase 1 (CES1) is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. These enzymes have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They are major determinants of the pharmacokinetic behavior of most therapeutic agents containing an ester or amide bond. CES1 shares the serine hydrolase fold observed in other esterases. CES1 hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. CES1 participates in detoxification of drugs such as cocaine and heroin in serum and liver. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds.
Sequence: His19-Glu562
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 61.1 kDa
Observed MW: 60 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only