Recombinant Human Carboxypeptidase B2/CPB2 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032172-10, PKSH032172-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carboxypeptidase B2; CPB2
Target Synonym: Carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma Carboxypeptidase B; pCPB; Thrombin-Activable Fibrinolysis Inhibitor; TAFI; CPB2
UNIProt ID: Q96IY4
Research Areas: Signal Transduction; Cardiovascular; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q96IY4
Background: Carboxypeptidase B2 (CPB2) is a secreted enzyme that belongs to the peptidase M14 family. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen by the liver and circulates in the plasma as a plasminogen-bound zymogen. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides, such as kinins or anaphylatoxins, in the circulation regulating their activities. CPB2 also down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. CPB2 exhibits carboxypeptidase activity when it is activated by proteolysis at residue Arg92 of the thrombin/thrombomodulin complex. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Sequence: Phe23-Val423
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM ZnCl2, 10%Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.0 kDa
Observed MW: 58 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only