Recombinant Human Carboxypeptidase E/CPE Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032173-10, PKSH032173-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carboxypeptidase E; CPE
Target Synonym: Carboxypeptidase E(CPE for short); Carboxypeptidase H; Enkephalin convertase; Prohormone-processing carboxypeptidase
UNIProt ID: P16870
Research Areas: Signal Transduction; Neuroscience; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P16870
Background: The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. It is thought that membrane-associated CPE acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway. Defects in this protein are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.
Sequence: Arg42-Ser453
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM ZnCl2, 10%Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.1 kDa
Observed MW: 57 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only