Recombinant Human CD16a/FCGR3A Protein (176 Phe, His&AVI Tag), Biotinylated(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH030284-20, PKSH030284-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD16a; FCGR3A
Target Symptom: Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3; CD16; CD16A
Conjugation: Biotinylated
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His & AVI
UNIProt ID: P08637
Accession: P08637-1
Background: The Fc receptor with low affinity for IgG (FCGR3; or CD16) is encoded by 2 nearly identical genes; FCGR3A and FCGR3B; resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A; it is also known as CD16a; encodes a transmembrane protein expressed on activated monocytes/macrophages; natural killer (NK) cells; and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells; independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells; independent of antibody ligation.
Activity: Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a. The EC50 of Human CD16a is 2. 4 - 5. 7 μg/ml. 3. Labeling ratio of biotin to protein: 0.7-1.
Sequence: Met 1-Gln 208
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 25.1 kDa
Observed MW: 48 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only