Recombinant Human CD31/PECAM1 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031833-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD31; PECAM1
Target Symptom: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture; ELISA
Fusion Tag: C-His
Accession: EAW94208.1
Background: The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin α vβ 3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31-His coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately > 70% will adhere after 30 minutes at 37℃.
Sequence: Met 1-Lys 601
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 66 kDa
Observed MW: 100-110 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only