Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032245-10, PKSH032245-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Chitotriosidase; CHIT1
Target Synonym: Chitotriosidase-1; Chitinase-1; CHIT1; CHI3; CHIT; CHITD
UNIProt ID: Q13231
Research Areas: immunology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q13231
Background: Chitotriosidase-1 (CHIT1) is a glycoprotein that belongs to the Glycosyl Hydrolase 18 family and Chitinase class II subfamily. It is secreted by cultured macrophages and serves to degrade chitin, chitotriose and chitobiose. CHIT1 may participate in the defense against nematodes and other pathogens. It is highly expressed in the plasma of patients with Gaucher's disease type I, which can be used as diagnostic aid and to evaluate the success of treatment that brings levels back to normal. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly, respectively.
Sequence: Ala22-Asn466
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50.4 kDa
Observed MW: 54 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only