Recombinant Human Coagulation Factor IX/F9 Protein (His Tag) - PKSH032264

Recombinant Human Coagulation Factor IX/F9 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032264-10, PKSH032264-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Coagulation Factor IX; F9

Target Synonym: Coagulation factor 9; F9; Coagulation factor IX; Christmas factor; Plasma thromboplastin component; Coagulation factor IXa light chain; Coagulation factor IXa heavy chain; FIX; HEMB; P19; PTC; THPH8

UNIProt ID: P00740

Research Areas: Cardiovascular; immunology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P00740

Background: Coagulation factor IX（F9), is a member of the peptidase S1 family. It contains two EGF-like domains, a Gla domain and a peptidase S1 domain. It is primarily expressed in the liver and secreted in plasma. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa. Mutations in position 43 and 46 prevents cleavage of the propeptide, mutation in position 93 probably fails to bind to cell membranes, mutation in position 191 or in position 226 prevent cleavage of the activation peptide. Mutations of human F9 can result in thrombophilia and recessive X-linked hemophilia B (HEMB). An X-linked blood coagulation disorder characterized by a permanent tendency to hemorrhage, due to factor IX deficiency. It is phenotypically similar to hemophilia A, but patients present with fewer symptoms. Many patients are asymptomatic until the hemostatic system is stressed by surgery or trauma.

Sequence: Thr 29-Thr461

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 49.8 kDa

Observed MW: 90 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only