Product Description

Recombinant Human Complement Factor H/CFH Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032273-10, PKSH032273-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Complement Factor H; CFH

Target Synonym: AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HF; HF1; HF2; HUS

UNIProt ID: P08603

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P08603-2

Background: Complement Factor H (CFH) is a secreted protein which is a member of the regulators of complement activation family and is a complement control protein. It is expressed by the liver and secreted in plasma. Its principal function is to regulate the Alternative Pathway of the complement system, ensuring that the complement system is directed towards pathogens or other dangerous material and does not damage host tissue. Factor H regulates complement activation on self cells and surfaces by possessing both cofactor activity for the Factor I mediated C3b cleavage, and decay accelerating activity against the alternative pathway C3-convertase, C3bBb. Factor H exerts its protective action on self cells and self surfaces but not on the surfaces of bacteria or viruses, because it binds to glycosaminoglycans (GAGs) that are generally present on host cells but not, normally, on pathogen surfaces.

Sequence: Glu19-Leu449

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, 20%Glycerol, 5% Trehalose, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 50.0 kDa

Observed MW: 50 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only