Recombinant Human Creatine Kinase, Muscle/CKM Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032285-10, PKSH032285-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CKM
Target Synonym: Creatine kinase M-type; Creatine kinase M chain; M-CK; CKM; CKMM
UNIProt ID: P06732
Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P06732
Background: Creatine kinase M-type is also known as Creatine kinase M chain, M-CK. It is a protein that in humans is encoded by the CKM gene. It belongs to the ATP:guanido phosphotransferase family, containing 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. Creatine kinase M-type can reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. It plays a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence: Met 1-Lys381
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10%Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 44.1 kDa
Observed MW: 46 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only