Recombinant Human Cu/Zn Superoxide Dismutase, His (rHuCu/Zn SOD, His) - PR1013

Recombinant Human Cu/Zn Superoxide Dismutase, His (rHuCu/Zn SOD, His)

Catalogue Numbers: PR1013-20, PR1013-100, PR1013-1000

Sizes: 20µg, 100µg, 1.0mg (Contact us for the 1.0mg option)

Source: Escherichia coli

Molecular Weight:Approximately 20.0 kDa. a homodimer, non-glycosylated polypeptide chain containing 189 amino acids with 10 x His at the N-terminal.

Purity: >95% by SDS-PAGE and HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The potency per mg was tested by Pyrogallic Acid method and was found to be more than 10,000Units/mg.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.

AA Sequence: MGHHHHHHHH HHSSGHIEGR HMTYARAAAR QARALEATKA VCVLKGDGPV QGIINFEQKE SNGPVKVWGS IKGLTEGLHG FHVHEFGDNT AGCTSAGPHF
NPLSRKHGGP KDEERHVGDL GNVTADKDGV ADVSIEDSVI SLSGDHCIIG
RTLVVHEKAD DLGKGGNEES TKTGNAGSRL ACGVIGIAQ

Endotoxin: Less than 1EU/mg of rHuSOD, His as determined by LAL method.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China

Human Cu/Zn Superoxide Dismutase (SOD) catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide.