Recombinant Human CX3CL1/Fractalkine Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032289-10, PKSH032289-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CX3CL1; Fractalkine
Target Synonym: Fractalkine; C-X3-C Motif Chemokine 1; CX3C Membrane-Anchored Chemokine; Neurotactin; Small-Inducible Cytokine D1; CX3CL1; FKN; NTT; SCYD1
UNIProt ID: P78423
Research Areas: Cardiovascular; immunology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P78423
Background: Human Fractalkine (CX3CL1) is a member of the CX3C family of chemokines. Human Fractalkine contains both chemokine and mucin domain. The soluble form of Fractalkine is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane bound form of Fractalkine promotes leukocytes adhesion to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid). The mucin-like stalk permits it to bind to the cell surface.
Sequence: Gln25-Arg339
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 34.4 kDa
Observed MW: 70 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only