Recombinant Human/Cynomolgus VEGFA/VEGF165 Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH031978-20, PKSH031978-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Human; VEGFA; VEGF165
Target Symptom: MVCD1; VEGF; VEGF165; VPF; Vascular Endothelial Growth Factor Isoform 165
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: None
UNIProt ID: P15692
Accession: P15692-4
Background: Vascular endothelial growth factor (VEGF); also known as vascular permeability factor (VPF) and VEGF-A; is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis and endothelial cell growth; promoting cell migration; inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability; thus it was originally referred to as vascular permeability factor.
Activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 4-16 ng/mL.
Sequence: Met 1-Arg191
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19.2 kDa
Observed MW: 20 and 22 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only