Recombinant Human Cystatin 7/CST7 Protein (Fc Tag)(Active)
Size: 20μg
Catalogue Number: PKSH031813-20
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cystatin 7; CST7
Target Symptom: Cystatin-F; Cystatin-7; Cystatin-Like Metastasis-Associated Protein; CMAP; Leukocystatin; CST7
Research Area: Cell biology; Cardiovascular
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-hFc
Accession: NP_003641.3
Background: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.
Activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog#ES008). The IC50 value is < 5 nM.
Sequence: Met 1-His 145
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 41.3 kDa
Observed MW: 50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only