Recombinant Human Cytochrome b5/CYB5A Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032334-10, PKSH032334-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cytochrome b5; CYB5A
Target Synonym: Cytochrome b5; Microsomal Cytochrome b5 Type A; MCB5; CYB5A; CYB5
UNIProt ID: P00167
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P00167
Background: Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.
Sequence: Met 1-Asp134
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, pH 7.25.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.5 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only