Recombinant Human Deoxyribonuclease 1/DNASE1 protein (His tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMH100102-20, PDMH100102-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Deoxyribonuclease 1; DNASE1
Target Synonym: Deoxyribonuclease-1; EC 3.1.21.1; Deoxyribonuclease I; DNase I; Dornase alfa; DNASE1; DNL1; DRNI
UNIProt ID: P24855
Research Areas: Cell biology; Cancer; epigenetics and nuclear signal; metabolism
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P24855
Background: DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.
Sequence: Met 1-Lys282
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 30.91 kDa
Observed MW: 40 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only