Recombinant Human Diamine Oxidase/AOC1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032352-10, PKSH032352-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Diamine Oxidase; AOC1
Target Synonym: Amiloride-sensitive amine oxidase [copper-containing]; DAO; Diamine oxidase; Amiloride-binding protein 1; Amine oxidase copper domain-containing protein 1; Histaminase; Kidney amine oxidase; KAO; AOC1; ABP1; DAO1
UNIProt ID: P19801
Research Areas: Cell biology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P19801
Background: Amiloride-sensitive amine oxidase (AOC1) belongs to the copper/topaquinone oxidase family. The protein exists as homodimer by disulfide and mainly located in placenta and kidney. AOC1 catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. The activity of this protein can be inhibited by amiloride in a competitive manner. It is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels.
Sequence: Glu20-Val751
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 84.4 kDa
Observed MW: 90 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only