Recombinant Human DUSP3/VHR Protein (His & GST Tag)
Size: 100μg
Catalogue Numbers: PKSH031862-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: DUSP3; VHR
Target Synonym: Dual specificity protein phosphatase 3; DUSP3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR
UNIProt ID: P51452
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-His & GST
Accession: P51452
Background: Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP); and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs; VHR lacks mitogen-activated protein kinase (MAPK)-binding domain; and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates; it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and; therefore; may play a role in aspects of T lymphocyte physiology that depend on these kinases.
Sequence: Met 1-Pro 185
Purity: > 96 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 2mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 48.3 kDa
Observed MW: 44 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only