Recombinant Human ECH1 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033275-10, PKSH033275-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ECH1
Target Symptom: Delta(3; 5)-Delta(2; 4)-dienoyl-CoA isomerase; mitochondrial; ECH1;
Research Area: Signal Transduction; Tags & Cell Markers; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
UNIProt ID: Q13011
Accession: Q13011
Background: Human delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase(ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.
Sequence: Thr34-Leu328
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 34.5 kDa
Observed MW: 30-35 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only