Recombinant Human EphA3 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031116-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: EphA3
Target Symptom: EK4; ETK; ETK1; HEK; HEK4; TYRO4
Research Area: Signal Transduction; Cardiovascular; Neuroscience;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_005224.2
Background: EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Activity: Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6. 2-14.6 ng/ml.
Sequence: Met 1-Gln541
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 60.3 kDa
Observed MW: 65-70 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only