Recombinant Human Ephrin-A4/EFNA4 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP00143-10, RP00143-20, RP00143-50, RP00143-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Ephrin-A4/EFNA4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu26-Gly171) of human Ephrin-A4/EFNA4 (Accession # NP_005218.1) fused with an Fc, 6×His tag at the C-terminus.
Alternate Names: EFL4, EPLG4, LERK4, EFNA4
SWISS: P52798
GeneID: 1945
Species: Human
Purity: ≥ 85% as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: EPH-related receptor tyrosine kinase ligand 4 (Ephrin-A4) also known as EFNA4, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A4/EFNA4 functions as a cell surface GPI-bound ligand for Eph receptor, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Human EphA7 at 0.5 μg/mL (100 μL/well) can bind Human EFNA4 with a linear range of 0.039-0.942 ng/mL.
Source: HEK293 cells
Tag: C-hFc&His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: LRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERKSESAHPVGSPGESG
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only