Recombinant Human FBPase 1/FBP1 Protein (E. coli, His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033276-10, PKSH033276-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: FBPase 1; FBP1
Target Symptom: Fructose-1; 6-Bisphosphatase 1; FBPase 1; D-Fructose-1; 6-Bisphosphate 1-Phosphohydrolase 1; FBP1; FBP
Research Area: Signal Transduction; Cancer; epigenetics and nuclear signal; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
UNIProt ID: P09467
Accession: P09467
Background: Fructose-1; 6-Bisphosphatase 1 (FBPase 1) is a member of the FBPase class 1 family. FBPase 1 is a gluconeogenesis regulatory protein; which catalyzes the hydrolysis of fructose 1; 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. FBPase 1 can assume an active R-state; or an inactive T-state. FBPase 1 deficiency is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis in newborn infants or young children. FBPase 1 coupled with phosphofructokinase (PFK) is involved in the metabolism of pancreatic islet cells.
Sequence: Ala2-Gln338
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 1mM EDTA, 20% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 37.9 kDa
Observed MW: 38 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only