Recombinant Human FGF-12/FGF12 Protein (Active)
Size: 50μg
Catalogue Number: PKSH032433-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: FGF-12; FGF12
Target Symptom: Fibroblast Growth Factor 12; FGF-12; Fibroblast Growth Factor Homologous Factor 1; FHF-1; Myocyte-Activating Factor; FGF12; FGF12B; FHF1
Target Species: Human
Expression Host: E.coli
Application: ELISA
Fusion Tag: None
UNIProt ID: P61328
Accession: P61328-2
Background: Fibroblast Growth Factor 12 (FGF-12) is a member of the fibroblast growth factor (FGF) family. FGF-12 is probably involved in nervous system development and function. FGF-12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfectedinto mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
Activity: Immobilized Human FGF-12 at 2ug/ml(100 μl/well) can bind Human FGFR3-Fc(Cat: PKSH033678). The ED50 of Human FGF-12 is 1. 27 ug/ml.
Sequence: Met 1-Thr181
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5mM EDTA, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.5 kDa
Observed MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only