Recombinant Human FGF-7/KGF Protein (Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032444-10, PKSH032444-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: FGF-7; KGF
Target Symptom: Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF
Research Area: Signal Transduction; Cancer;
Target Species: Human
Expression Host: E.coli
Application: ELISA
Fusion Tag: None
UNIProt ID: P21781
Accession: P21781
Background: Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities; and are involved in a variety of biological processes; including embryonic development; cell growth; morphogenesis; tissue repair; tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor; whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.
Activity: Immobilized Human KGF at 2μg/ml(100 μl/well) can bind Human FGF R3-Fc(Cat: PKSH033678). The ED50 of Human KGF is 0.788 ug/ml.
Sequence: Cys32-Thr194
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; pH8.0; l50mM NaCl.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19.1 kDa
Observed MW: 23&38 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only