Recombinant Human Fibronectin/FN Protein (Active)
Size: 50μg
Catalogue Number: PKSH032450-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Fibronectin; FN
Target Symptom: Fibronectin; FN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF
Research Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P02751
Accession: P02751
Background: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains; fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II; and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.
Activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
Sequence: Pro1270-Ser1546&Ala1721-Thr2016
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 6.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 62.7 kDa
Observed MW: 60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only