Recombinant Human GADD45A/DDIT-1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033661-10, PKSH033661-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: GADD45A; DDIT-1
Target Symptom: Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha; DNA Damage-Inducible Transcript 1 Protein; DDIT-1; GADD45A; DDIT1; GADD45
Research Area: epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
UNIProt ID: P24522
Accession: P24522
Background: Growth Arrest and DNA Damage-Inducible Protein GADD45 α (GADD45A) is a member of the GADD45 family. GADD45A can be induced by UV irradiation; X-rays; growth arrest and alkylating agents; of which can be mediated by some kinases other than PKC. GADD45A can interact with MAPK14; GADD45GIP1; PCNA. In T-cells; GADD45A functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. GADD45A may affect PCNA interaction with some cell division protein kinase complexes; stimulating DNA excision repair in vitro and inhibits entry of cells into S phase.
Sequence: Met1-Arg165
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.5 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only