Recombinant Human Galectin-3/LGALS3 Protein, Low Endotoxin(Active)
Size: 100μg
Catalogue Number: PKSH031707-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Galectin-3; LGALS3
Target Symptom: Galectin-3; Gal-3; 35 kDa Lectin; Carbohydrate-Binding Protein 35; CBP35; Galactose-Specific Lectin 3; Galactoside-Binding Protein; GALBP; IgE-Binding Protein; L-31; Laminin-Binding Protein; Lectin L-29; Mac-2 Antigen; LGALS3; MAC2; P35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2
Research Area: Cell biology; Neuroscience
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P17931
Accession: P17931
Background: Leukotriene A-4 hydrolase; also known as LTA-4 hydrolase; Leukotriene A (4) hydrolase; LTA4H and LTA4; is cytoplasm protein which belongs to thepeptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs; arachidonic acid was converted into the unstable intermediate epoxide LTA4; which may in turn be conjugated with glutathione to form the spasmogenic LTC4; or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium; and also modulates immune responses. As a bifunctional zinc metalloenzyme; LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
Activity: Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 3-15 μg/ml.
Sequence: Met 1-Ile 250
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.005 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.1 kDa
Observed MW: 30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only