Recombinant Human Gankyrin/PSDM10 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032479-10, PKSH032479-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Gankyrin; PSDM10
Target Synonym: 26S Proteasome Non-ATPase Regulatory Subunit 10; 26S Proteasome Regulatory Subunit p28; Gankyrin; p28(GANK); PSMD10
UNIProt ID: O75832
Research Areas: Cell biology; Cancer
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
Accession: O75832
Background: Gankyrin is a multicatalytic proteinase oncoprotein consists of 7 ankyrin repeats. Gankyrin overexpressed in most hepatocellular carcinomas. Gankyrin is involved in theregulation of the phosphorylation of the retinoblastoma protein by CDK4 to enhance the ubiquitinylation of p53 by MDM2. Gankyrin is also involved in progression of esophageal squamous cell carcinoma. Gankyrin plays an oncogenic role especially in early stages of human epatocarcinogenesis.
Sequence: Met 1-Gly226
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 24.4 kDa
Observed MW: 26 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only