Recombinant Human GAS-6 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP02964-10, RP02964-20, RP02964-50, RP02964-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human GAS-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ala678) of Human GAS-6(Accession #NP_000811) fused with a His tag at the C-terminus.
Alternate Names: GAS6, AXLLG, Growth arrest-specific protein 6, GAS-6, AXL receptor tyrosine kinase ligand
SWISS: Q14393-2
GeneID: 2621
Species: human
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: GAS-6 is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG). Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors. Gas6 induces a variety of responses, including prevention of apoptosis , cell proliferation , platelet-mediated thrombosis , retinal epithelial cell phagocytosis of outer rod segments , inhibition of VEGF-induced endothelial cell chemotaxis , and the differentiation and expansion of NK cell precursors. The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells. Several of these effects have been shown to require gamma -carboxylation of the Gla domain.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: MAPSLSPGPAALRRAPQLLLLLLAAECALAALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only