Recombinant Human GSS Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032497-10, PKSH032497-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: GSS
Target Synonym: Glutathione Synthetase; GSH Synthetase; GSH-S; Glutathione Synthase; GSS
UNIProt ID: P48637
Research Areas: Signal Transduction; Cell biology; Neuroscience; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: P48637
Background: Glutathione Synthetase belongs to the eukaryotic GSH synthase family. Glutathione Synthetase is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine to form glutathione. Glutathione play an important role in a variety of biological functions, including detoxification of xenobiotics, protection of cells from oxidative damage by free radicals, and membrane transport. The protein functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in Glutathione Synthetase can also cause the glutathione synthetase deficiency of erythrocytes, which is a mild form causing hemolytic anemia.
Sequence: Ala2-Val474
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 53.5 kDa
Observed MW: 55 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only