Recombinant Human HMGB1 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033654-10, PKSH033654-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: HMGB1
Target Symptom: High Mobility Group Protein B1; High Mobility Group Protein 1; HMG-1; HMGB1; HMG1
Research Area: epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
UNIProt ID: P09429
Accession: P09429
Background: High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.
Sequence: Met1-Phe89
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 50mMHepes; 500mMNaCl; 0.5mMDTT, pH7.9 .
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 10.5 kDa
Observed MW: 14 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only