Recombinant Human HSP27/HSPB1 Protein (His Tag)
Size: 100μg
Catalogue Number: PKSH031656-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: HSP27, HSPB1
Target Synonym: CMT2F, HEL-S-102, HMN2B, HS.76067, Hsp25, HSP27, HSP28, SRP27
Research Areas: Signal Transduction, Cardiovascular, Cancer,
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-His
Accession: NP_001531.1
Background: HSP27 is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3.
Sequence: Met 1-Lys205
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM HEPES, 0.1M KCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 28 kDa
Observed MW: 28 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only