Recombinant Human IFN-alpha I/IFN17 Protein
Sizes: 10ug, 20ug, 50ug
Catalogue Numbers: RP01899-10, RP01899-20, RP01899-50
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human IFN-alpha I/IFN17 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Asp189) of Human IFN-alpha I/IFN17 (Accession #NP_067091.1) fused with a His tag at the C-terminus.
Alternate Names: Interferon alpha-17, IFN-alpha-17, Interferon alpha-88, Interferon alpha-I', LeIF I, Interferon alpha-T, IFNA17
SWISS: P01571
GeneID: 3451
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors. Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology. Structurally, type I IFNs belong to the class of five helicalbundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds. There is not a mouse homolog for IFNA17, but mature human IFNA17 shares 58% aa sequence identity with chimpanzee IFNA17. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit). Individual IFNA subtypes are known to display unique efficacies to viral protection. IFNA17 has been shown to be potent against HIV-1 activity. Human IFNA17 is the only IFNA subtype identified with antiviral activity but a reduced ability to activate NK cells. A mutation in IFNA17, Ile184Arg, is associated with an increased risk for cervical cancer.
Bio-Activity: Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.17‑0.68 ng/mL, corresponding to a specific activity of 1.47×10^6 ~5.88×10^6 units/mg.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: CDLPQTHSLGNRRALILLAQMGRISPFSCLKDRHDFGLPQEEFDGNQFQKTQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNNLEACVIQEVGMEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKILRRKD
Endotoxin: < 0.01 EU/μg of the protein by LAL method
Research Use Only