Recombinant Human IL-18 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100217-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-18
Target Synonym: IGIF; IL-18; IL-1g; IL1F4; Interleukin 18; IL18; IGIF
UNIProt ID: Q14116
Research Areas: Cancer; immunology; metabolism
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-His
Accession: Q14116
Background: Interleukin-18 (IL-18; also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. IL-18 is an IL-1− like cytokine that requires cleavage with caspase-1 to become active; was found to increase IgE production in a CD4+ T cells-; IL-4− and STAT6− dependent fashion. IL-18 and T cell receptor− mediated stimulation could induce naï ve CD4+ T cells to develop into IL-4− producing cells in vitro. Thus; caspase-1 and IL-18 may be critical in regulation of IgE production in vivo; providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was; in turn; upregulated by TNF-α and IL-1β; suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides; synergistic combinations of IL-18; IL-12; and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
Sequence: Tyr 37-Asp 193
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 17.16 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only