Recombinant Human IL-21 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01708-10, RP01708-20, RP01708-50, RP01708-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human IL-21 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Ser162) of human IL-21 (Accession #NP_068575.1) fused with no additional amino acid.
Alternate Names: Za11; IL-21; CVID11; IL21
SWISS: Q9HBE4
GeneID: 59067
Species: Human
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation).
Bio-Activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2.7-10.8 ng/mL, corresponding to a specific activity of 9.3×10^4 ~3.7×10^5 units/mg.
Source: HEK293 cells
Tag: No-tag
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: QDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only