Recombinant Human IL-23 alpha & Mouse IL-12 beta Heterodimer (C-6His)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041396-10, PKSM041396-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-23A & IL-12B
Target Symptom: SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2
Research Area: Microbiology; Cancer; immunology
Target Species: Human & Mouse
Expression Host: HEK293 Cells
Fusion Tag: C-6His
UNIProt ID: Q9NPF7&P43432
Accession: Q9NPF7&P43432
Background: Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
Sequence: Arg20-Pro189&Met23-Ser335
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 56.9 kDa
Observed MW: 65-80 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only