Recombinant Human Interleukin-13/IL-13 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032617-10, PKSH032617-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-13; IL-13
Target Symptom: ALRH; IL-13; P600; IL13
Research Area: Cardiovascular; Cancer; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: C-6His
UNIProt ID: P35225
Accession: P35225
Background: Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.
Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1. 5-4. 5 ng/ml.
Sequence: Leu25-Asn146
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14.3 kDa
Observed MW: 13 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only