Recombinant Human Interleukin-17F/IL-17F Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032624-10, PKSH032624-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-17F; IL-17F
Target Symptom: Interleukin-17F; IL-17F; Cytokine ML-1; Interleukin-24; IL-24; IL17F; IL24
Research Area: Cardiovascular; Cancer; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: C-6His
UNIProt ID: Q96PD4
Accession: Q96PD4
Background: Interleukin-17F (IL-17F) exists in a disulfide-linked heterodimer that belongs to the IL-17 family. IL-17F is expressed in activated; but not resting; CD4+ T-cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines; including IL-6; IL-8; and granulocyte colony-stimulating factor. IL-17F can regulate cartilage matrix turnover and stimulates PBMC and T-cell proliferation. IL-17F is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2; TGFB1/TGFB; and monocyte chemoattractant protein-1. Defects in IL-17F are the cause of familial candidiasis type 6 (CANDF6).
Activity: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 21.87 ng/ml.
Sequence: Arg31-Gln163
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.0 kDa
Observed MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only