Recombinant Human Interleukin-2/IL-2 Protein (E.coli, Active)
Size: 100μg
Catalogue Number: PKSH033425-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-2; IL-2
Target Symptom: Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL5
Research Area: immunology
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P60568
Accession: P60568
Background: Interleukin-2(IL-2) is an interleukin; a type of cytokine signaling molecule in the immune system; belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells; NK cells; lymphokine-activated killer cells; monocytes; macrophages and oligodendrocytes.
Activity: 1. Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is < 0.2 ng/mL The specific activity of recombinant human IL-2 is approximately > 2. 5 x10^7 IU/mg.
2. Measure by its ability to induce proliferation in NK cells. The ED50 for this effect is < 46ng/mL.
Sequence: Ala21-Thr153
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH 4.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 15.5 kDa
Observed MW: 14 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only