Recombinant Human Interleukin-2/IL-2 Protein (Mutant)(Active)
Size: 50μg
Catalogue Number: PKSH033625-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-2; IL-2
Target Symptom: Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL3
Research Area: immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P60568
Accession: P60568
Background: Interleukin-2(IL-2) is an interleukin; a type of cytokine signaling molecule in the immune system; belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells; NK cells; lymphokine-activated killer cells; monocytes; macrophages and oligodendrocytes. New research has shown that IL-2 mutant reduced toxicity while being more potent at stimulating anti-tumor effector immune cells.
Activity: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The specific activity of Recombinant Human IL-2 is ≥1×107 IU/mg.
Sequence: Ala21-Thr153(L100F; R101D; L105V; I106V; I112F)
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.5 kDa
Observed MW: 16&18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only