Recombinant Human LCAT Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032685-10, PKSH032685-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LCAT
Target Synonym: Phosphatidylcholine-sterol acyltransferase; also named Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase and LACT; is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.
UNIProt ID: P04180
Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P04180
Background: Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes, followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).
Sequence: Phe25-Glu440
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Acetate Buffer pH-4.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 48.1 kDa
Observed MW: 66 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only