Recombinant Human LDLR Protein (His Tag/AVI)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH032711-20, PKSH032711-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: LDLR
Target Synonym: Low-Density Lipoprotein Receptor; LDL Receptor; LDLR; FH; FHC; LDL R; LDL Receptor; LDLCQ2
UNIProt ID: P01130
Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;
Conjugation: Biotinylated
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His-AVI
Accession: P01130
Background: Low-density lipoprotein receptor 9 (LDL receptor) is a single-pass type I membrane protein which belongs to the LDLR family. It contains 3 EGF-like domains, 7 LDL-receptor class A domains, and 6 LDL-receptor class B repeats. This protein binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR will result in familial hypercholesterolemia.
Sequence: Ala22-Arg788
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 90.1 kDa
Observed MW: 137 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only