Recombinant Human Legumain/LGMN Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032687-10, PKSH032687-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Legumain; LGMN
Target Synonym: Legumain; Asparaginyl Endopeptidase; Protease Cysteine 1; LGMN; PRSC1
UNIProt ID: Q99538
Research Areas: Signal Transduction; Cell biology; Cancer; immunology; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q99538
Background: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.
Sequence: Ile18-Tyr433
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 48.7 kDa
Observed MW: 56 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only