Recombinant Human LRAP/ERAP2 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031454-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LRAP; ERAP2
Target Symptom: FLJ23633; FLJ23701; FLJ23807; L-RAP; LRAP; LRAP.ERAP2
Research Area: Cell biology; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: N-His
Accession: NP_071745.1
Background: Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.
Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is > 50 pmoles/min/μg.
Sequence: Ala 56-Thr 960
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 106 kDa
Observed MW: 115-125 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only