Recombinant Human MASP1 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100170-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: MASP1
Target Synonym: Mannan-Binding Lectin Serine Protease 1; Complement Factor MASP-3; Mannose-Binding Lectin-Associated Serine Protease 1; MASP-1; RaRF; Serine Protease 5; MASP1; CRARF; CRARF1; PRSS5
UNIProt ID: P48740
Research Areas: immunology
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P48740
Background: Mannan-Binding Lectin Serine Protease 1 (MASP-1) belongs to the peptidase S1 family. MASP1 contains two CUB domains, one EGF-like domain, one peptidase S1 domain and two Sushi (CCP/SCR) domains. MASP1 is primarily expressed in liver. MASP1 involved in the lectin pathway of the complement, performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. MASP1 is synthesized as a zymogen and activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. MASP1 is not directly involved in complement activation but may act as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP2. MASP1 is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. MASP1 is inhibited by SERPING1 and A2M.
Sequence: His 20-Ala 447
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 46.97 kDa
Observed MW: 48 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only