Recombinant Human MERTK/MER Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033481-10, PKSH033481-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: MERTK; MER
Target Symptom: Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; MERTK; MER; Mer
Research Area: Signal Transduction; Cell biology; Neuroscience; immunology; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
UNIProt ID: Q12866
Accession: Q12866
Background: Tyrosine-protein kinase Mer (MERTK) is a single-pass type I membrane protein which belongs to the MER/AXL/TYRO3 receptor kinase family. MERTK include two fibronectin type-III domains; two Ig-like C2-type domains; and one tyrosine kinase domain. It can’t be expressed in normal B- and T-lymphocytes; but it is usually expressed in numerous neoplastic B- and T-cell lines. MERTK could regulate many physiological processes; such as cell survival; migration; differentiation. It was demonstrated that the MERTK plays critical role in the engulfment and efficient clearance of apoptotic cells; platelet aggregation; and cytoskeleton reorganization. Not only these; it also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1; which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. In addition; MERTK could regulate rod outer segments fragments phagocytosis in the retinal pigment epithelium (RPE); deficiency in MERTK are the cause of retinitis pigmentosa.
Sequence: Met177-Ala499
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris; 150mM NaCl; pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 36 kDa
Observed MW: 60-120 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only