Recombinant Human MICB Protein (His Tag)(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMH100004-20, PDMH100004-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: MICB
Target Symptom: PERB11.2
Research Area: immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
UNIProt ID: Q29980
Accession: Q29980
Background: MHC class I polypeptide-related sequence B; also known as MICB; is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA; a closely related protein; both of which contain three extracellular immunoglobulin-like domains; but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen; binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells; CD8+αβ T cells; and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells; but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance; viral infections; and autoimmune diseases.
Activity: Immobilized human MICB-His at 10 μg/ml (100 μl/well) can bind human NKG2D, The EC50 of human NKG2D is 0.52-1. 2 μg/ml.
Sequence: Met 1-Gly 298
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33 kDa
Observed MW: 45-50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only